%0 Journal Article
%T Cyclin-dependent kinase 5-mediated phosphorylation of chloride intracellular channel 4 promotes oxidative stress-induced neuronal death
%A Dong Guo
%A Guimiao Chen
%A Guojun Bu
%A Huifang Li
%A Jie Zhang
%A Jiechao Zhou
%A Maoqiang Xue
%A Pan Xiong
%A Siqi Wang
%A Wenting Xie
%A Ye Zhang
%A Yuehong Gao
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-018-0983-1
%X a Immunoblotting of proteins pulled down by glutathione Sepharose from lysates of N2a cells transiently transfected with GST alone or GST-CLIC4 as indicated. Membranes were probed with antibodies against the indicated proteins. b Immunoblotting of proteins pulled down by glutathione Sepharose from lysates of N2a cells transiently transfected with HA-CLIC4 and GST alone or GST-CLIC4. Membranes were probed with antibodies to the indicated proteins. c Immunoblotting of proteins immunoprecipitated from brain homogenates of wild-type C57BL/6 mice with antibodies against normal IgG or anti-CLIC4 antibody. d Immunoblotting of proteins immunoprecipitated from brain homogenates of wild-type C57BL/6 mice with antibodies against normal IgG, anti-CDK5 or anti-p35 antibody. e¨Cg Immunofluorescence staining of DIV7 primary cortical neurons with anti-CDK5 and anti-CLIC4 antibodies showed the co-localization of CDK5 and CLIC4. Higher magnification around neuronal soma (f) and neurite (g) of the indicated area of e was presented and the co-localization was pointed with arrows. DAPI is a nucleus dye. Scale bar£¿=£¿10£¿¦Ìm. h BiFC fluorescence and immunostaining showed that CDK5 and CLIC4 interacted in the cytoplasm of N2a cells. N2a cells were transfected with VN173-CDK5 and VC155 vector or VC155-CLIC4, then immunostained with anti-GFP antibody. DAPI is a nucleus dye. Scale bar£¿=£¿10£¿¦Ì
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6147799/