%0 Journal Article
%T NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR
%A En Xu
%A Feng Wang
%A Hong Chen
%A Ji Miao
%A Linsen Shi
%A Shangce Du
%A Shichao Ai
%A Wenxian Guan
%A Xiaofeng Lu
%A Xing Kang
%A Xuefeng Xia
%A Zhouting Zhu
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-018-0953-7
%X a ROS generation by adherent or suspension MKN-45 and AGS cultures was measured by flow cytometry using carboxy-H2DCFDA. The solution that contains GC cells without treatment using ROS-sensitive substrate H2DCFDA was used as the negative control. b ROS generation by MKN-45 and AGS suspension cultures treated with H2O or NAC was measured by flow cytometry using carboxy-H2DCFDA. c Anoikis rates of MKN-45 and AGS cells treated with H2O or NAC in suspension conditions for 24£¿h were measured by flow cytometry using Annexin V-FITC. ***P£¿<£¿0.001. d MKN-45 and AGS cells were treated with exogenous H2O2 (0, 0.1, 0.5, 1, 2, 5, 10, 50, 100£¿¦ÌM), and the rate of anoikis detected by flow cytometry using Annexin V-FITC. e Cellular morphology of MKN-45 and AGS suspension cells treated with H2O or NAC captured by microphotography after 24£¿h of culture (magnification ¡Á100). f The average number of aggregated MKN-45 and AGS suspension cells treated with H2O or NAC in each field was measured after 24£¿h of culturing. **P£¿<£¿0.01. All experiments were performed three independent time
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148243/