%0 Journal Article
%T Potent effect of the MDM2 inhibitor AMG232 on suppression of glioblastoma stem cells
%A Do-Hyun Nam
%A Gyu Ha Ryu
%A Hee Jin Cho
%A Jeong-Woo Oh
%A Nam-Gu Her
%A Suji Han
%A Yeri Lee
%A Yun Jeong Oh
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-018-0825-1
%X a Chemical structures of RG7112 and AMG232. b, c Cell numbers of U373MG, LN18, U251MG, A1207, DBTRG-05MG, and U87MG cells treated with different concentrations (0.07¨C54£¿¦ÌM) of RG7112 or AMG232 for 72£¿h were evaluated by quantifying image-based cell counting. Nonlinear regression analyses of dose£¿response curves are shown (n£¿=£¿3). Error bars represent standard deviation (SD). d The IC50 values obtained from dose£¿response curves are shown. Each bars represent mean (n£¿=£¿3) and 95% confidence interval (CI). e IC50 values of TP53 mutant cell lines (n£¿=£¿3) and TP53 wild-type cell lines (n£¿=£¿3) to RG7112 and AMG232 are shown. Data represent the mean and SD error (*p£¿<£¿0.01). f An image-based immunofluorescence assay for p21 (green), p53 (red) and DAPI (blue) in A1207 cells treated with DMSO, RG7112 (2£¿¦ÌM), AMG232 (2£¿¦ÌM), and Camptothecin (10£¿¦ÌM) for 72£¿h are shown. g p21 fluorescence intensity measurements for A1207 cells treated with increasing concentrations of RG7112 or AMG232 for 72£¿h are shown. The Z-factor for 18£¿¦ÌM RG7112 and 18£¿¦ÌM AMG232 in A1207 cells are indicated. h The mean percentage of p21-positive cells (n£¿=£¿3, SD) for six glioblastoma cell lines treated with DMSO, RG7112 (2£¿¦ÌM), AMG232 (2£¿¦ÌM) for 72£¿h are show
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052082/