%0 Journal Article
%T Bone morphogenetic protein 2 promotes human trophoblast cell invasion by upregulating N-cadherin via non-canonical SMAD2/3 signaling
%A Christian Klausen
%A Hong-Jin Zhao
%A Hua Zhu
%A Peter C. K. Leung
%A Yan Li
%A Yan-Ling Wang
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-017-0230-1
%X BMP2 increases HTR8/SVneo and primary human EVT cell invasion. a, b HTR8/SVneo cells were treated with vehicle (Ctrl) or BMP2 (25 or 50£¿ng/mL) and cell invasiveness was examined by Matrigel-coated transwell assay. Representative images of the invasion assay ((a); scale bar 100£¿¦Ìm) and summarized quantitative results (b) are shown separately (n£¿=£¿3). c HTR8/SVneo cells were seeded in 24-well plates and treated every 24£¿h with vehicle or BMP2 (25 or 50£¿ng/mL) for a total of 72£¿h. Cell viability was determined by MTT assay at 24, 48, and 72£¿h after BMP2 treatment (n£¿=£¿3). d, e Primary human EVT cells were treated with or without 25£¿ng/mL BMP2 and cell invasiveness was examined by Matrigel-coated transwell assay. Representative images of the invasion assay ((d); scale bar 100£¿¦Ìm) and combined quantitative results (e) are shown separately (n£¿=£¿5). Results are displayed as the mean£¿¡À£¿SEM of at least three independent experiments and values without common letters are significantly different (P£¿<£¿0.05
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833391/