%0 Journal Article
%T Pharmacological activation of autophagy favors the clearing of intracellular aggregates of misfolded prion protein peptide to prevent neuronal death
%A Aldo Pagano
%A Alessandro Corsaro
%A Beatrice Scoti
%A Carlo Tacchetti
%A Carola Porcile
%A Claudio Russo
%A Katia Cortese
%A Maria Cristina Gagliani
%A Mario Nizzari
%A Stefano Thellung
%A Tullio Florio
%A Valentina Villa
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-017-0252-8
%X a Flow cytometry analysis of cell death. Dead cells in the culture were quantified after 24 and 48£¿h of treatment with PBS (vehicle) or PrP90-231 (100 and 500£¿nM), by measuring the percent of cells that incorporate SYTOX green. * P£¿<£¿0.05 vs. vehicle. b Cell viability was quantified in control cells after 48 and 72£¿h (treatment with PBS, 0 on abscissae) or PrP90-231 (1 and 5£¿¦ÌM) measuring the percent of live cells by Trypan blue exclusion test. *P£¿<£¿0.05 and **P£¿<£¿0.01 vs. PBS. c Mitochondrial viability of A1 cells was evaluated by MTT-reduction assay after 72£¿h of treatment with PBS (0 on abscissae) and PrP90-231 at concentrations ranging from 0.1 to 10£¿¦ÌM. *P£¿<£¿0.05 and **P£¿<£¿0.01 vs. PBS. d Phase-contrast pictures of A1 cells that have been treated with PrP90-231 5£¿¦ÌM for two (middle panel) and four (lower panel) days in comparison with untreated (upper panel). Images has been acquired with C- plan 10£¿¡Á£¿/0.22 magnification lens. Bar 100£¿¦Ìm. All the data are reported as mean£¿+£¿/£¿ SEM of three independent experiments each performed in quadruplicat
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833808/