%0 Journal Article
%T Involvement of both caspase-8 and Noxa-activated pathways in endoplasmic reticulum stress-induced apoptosis in triple-negative breast tumor cells
%A Abelardo L¨®pez-Rivas
%A Ana Cano-Gonz¨¢lez
%A Daniel Iglesias-Serret
%A Joan Gil
%A Marta Mauro-Lizcano
%J Archive of "Cell Death & Disease".
%D 2018
%R 10.1038/s41419-017-0164-7
%X Apoptosis was measured after 3 days of treatment by subG1 analysis as described under Materials and methods section. Error bars represent standard error of the mean (SEM) from at least three independent experiments. *P£¿<£¿0.05; **P£¿<£¿0.01; ***P£¿<£¿0.001. A) Dose¨Cresponse of thapsigargin (TG) in apoptosis in TNBC (red lines) and non-TNBC (blue lines) cells. B) Kinetics of apoptosis induction by TG (100£¿nM) in TNBC (MDA-MB231 and BT549) and non-TNBC (T47D) cell lines. C) MDA-MB231 and T47D were treated with TG (100£¿nM) for the indicates times. Kinetics of caspase processing and Bid loss were determined in whole-cell extracts by western blotting. Tubulin levels were used as protein-loading controls. D) Apoptosis was assessed in Bcl-XL-overexpressing MDA-MB231 cells treated with 100£¿nM TG or 1£¿¦Ìg/ml tunicamycin (TN). Bcl-XL expression was assessed by western blotting. Tubulin expression was used as a protein-loading contro
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833688/