%0 Journal Article
%T Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture
%A Eef Parthoens
%A Hanne Van Gorp
%A Mohamed Lamkanfi
%A Nathalia M. de Vasconcelos
%A Nina Van Opdenbosch
%J Archive of "Cell Death and Differentiation".
%D 2019
%R 10.1038/s41418-018-0106-7
%X Cell detachment and blebbing during necroptosis and pyroptosis. a B6Nlrp1b+ BMDMs were stimulated with TNF+BV6+zVAD-fmk (TBz: 20 ng/ml, 2 ¦ÌM and 50 ¦ÌM, respectively) and imaged in culture media containing Sytox Green. b The rCTB-stained B6Nlrp1b+ BMDMs were stimulated with LeTx and imaged as in (a). a, b Confocal images were acquired every 3£¿min. c, d B6Nlrp1b+ BMDMs pretreated with Y27632 (10£¿¦ÌM) (c) or (£¿)-blebbistatin (10£¿¦ÌM) (d) were stimulated with LeTx or TBz and imaged. Fluorescent micrographs show the maximum intensity projection (Sytox Green) or the single plane (rCTB) of a representative cell from 3 independent experiments (TBz n£¿=£¿30; LeTx n£¿=£¿30; LeTx+Y27632 n£¿=£¿25; TBz+Y27632 n£¿=£¿23; LeTx+(£¿)-blebbistatin n£¿=£¿19; TBz+(£¿)-blebbistatin n£¿=£¿20). In all panels, time point zero indicates the first detection of Sytox Green. All scale bars, 10£¿¦Ì
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294780/