%0 Journal Article
%T Constitutive IP3 signaling underlies the sensitivity of B-cell cancers to the Bcl-2/IP3 receptor disruptor BIRD-2
%A Ann Janssens
%A Claudio Martines
%A Dimitar G. Efremov
%A Geert Bultynck
%A Giampaolo Morciano
%A Haidar Akl
%A Humbert De Smedt
%A Jan B. Parys
%A Kathia Dubron
%A Katja Rietdorf
%A Katsuhiko Mikoshiba
%A Kirsten Welkenhuyzen
%A Luca Laurenti
%A Mart Bittremieux
%A Martin D. Bootman
%A Myriam Baes
%A Paolo Pinton
%A Peter Vandenberghe
%A Rita M. La Rovere
%J Archive of "Cell Death and Differentiation".
%D 2019
%R 10.1038/s41418-018-0142-3
%X High IP3R2-expression levels are not sufficient per se to render cells sensitive to BIRD-2. a The IP3R2- and Bcl-2-protein levels present in cell lysates from SU-DHL-4 (40£¿¦Ìg), OCI-LY-1 (40£¿¦Ìg), and HepG2 (40£¿¦Ìg) cells and from microsomes extracted from primary hepatocytes (20£¿¦Ìg) were determined by western-blot analysis. The expression level of calnexin was used as a control for equal loading. b Representative dot plots from flow cytometry analysis measuring apoptosis by staining SU-DHL-4, OCI-LY-1, HepG2 cells and primary hepatocytes with Annexin V-FITC and 7-AAD. Cells were treated with vehicle or 10£¿¦ÌM BIRD-2 for 2£¿h. The dot plots are representative of 3 independent experiments. c, d Quantitative analysis of 3 independent experiments detecting apoptosis in Annexin V-FITC/7-AAD-stained cells treated with vehicle or 10£¿¦ÌM BIRD-2. Apoptotic cell death was measured 2£¿h (c) and 24£¿h (d) after BIRD-2 treatment. Data are represented as average£¿¡À£¿SEM (N£¿=£¿3). Statistically significant differences were determined with a Student¡¯s t-test (paired, two-tailed, *P£¿<£¿0.05) (BIRD-2 versus vehicle
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370760/