%0 Journal Article
%T Glucocorticoid receptor in astrocytes regulates midbrain dopamine neurodegeneration through connexin hemichannel activity
%A Anne-Claire Compagnion
%A Annette Koulakoff
%A Chenju Yi
%A Christian Giaume
%A Etienne C. Hirsch
%A Frank W Pfrieger
%A Fran£¿ois Tronche
%A Layal Maatouk
%A Luc Leybaert
%A Maria-Angeles Carrillo-de Sauvage
%A Pascal Ezan
%A Sheela Vyas
%A St¨¦phane Hunot
%J Archive of "Cell Death and Differentiation".
%D 2019
%R 10.1038/s41418-018-0150-3
%X Tamoxifen-dependent Cre expression, recombination and determination of GR deletion in GRCx30CreERT2 mice. a Representative panels showing Cre immunohistochemistry in GRCx30CreERT2 mice injected with either vehicle or tamoxifen, in cortex (cx), striatum, septum, substantia nigra (SN) and hippocampus. The small squares in tamoxifen-positive images are magnified in upper panels to show Cre recombinase. Scale bar, 200£¿¦Ìm. b Representative images showing mTomato (red) staining after vehicle injection and appearance of mGFP expression in astrocytes of striatum, substantia nigra (SN) and hippocampus after tamoxifen injections in Cx30CreERT2:mT/mG mice. Scale bar, 100£¿¦Ìm. c Representative images showing double labeling of GR (red) and S100¦Â (green) in cortex of GRloxp/loxP controls and GRCx30CreERT2 mice injected with tamoxifen. Scale bar, 5£¿¦Ìm. d, e Quantification of the percentage of S100¦Â +£¿astrocytes co-labeled with GR and (d) and NeuN£¿+£¿neurons expressing GR (e) in cortex (Cx), striatum (ST) and substantia nigra (SN) of controls and GRCx30CreERT2 mutant mice injected with tamoxifen. *p£¿<£¿0.05; **p£¿<£¿0.01, mutant vs control. Error bars represent SEM. n£¿=£¿4¨C5/grou
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370798/