%0 Journal Article
%T MPTP-driven NLRP3 inflammasome activation in microglia plays a central role in dopaminergic neurodegeneration
%A Boreum Hwang
%A Eunju Lee
%A Inhwa Hwang
%A Je-Wook Yu
%A Junghyun Son
%A Sangjun Park
%A Sujeong Hong
%A Yoeseph Cho
%J Archive of "Cell Death and Differentiation".
%D 2019
%R 10.1038/s41418-018-0124-5
%X NLRP3 deficiency attenuates motor dysfunctions and dopaminergic neuronal loss in MPTP-treated mice. a¨Cc Nlrp3+/+ or Nlrp3£¿/£¿ mice were administered with PBS or MPTP (40£¿mg/kg) for 5 days and then examined for motor activity impairment as described in the Methods. a The latent time to hold the bar in the string test 24£¿h post the final PBS or MPTP administration (Nlrp3+/+ mice, PBS (n£¿=£¿6), MPTP (n£¿=£¿10); Nlrp3£¿/£¿ mice, PBS (n£¿=£¿6), MPTP (n£¿=£¿11)). b The latent time to hold the bar in the catalepsy test 1£¿h post the final PBS or MPTP administration. (PBS (n£¿=£¿6), MPTP (n£¿=£¿11)). c The severity of hindlimb clasping in PBS- (n£¿=£¿6) or MPTP-treated (n£¿=£¿11) mice 1£¿h post the final PBS or MPTP administration. d Representative immunofluorescence image of the fixed brain sections containing SN of PBS- or MPTP-treated Nlrp3+/+ or Nlrp3£¿/£¿ mice after staining with anti-TH antibody (green). DAPI represents the nuclear signal (blue). Scale bars, 200£¿¦Ìm. e Quantification of relative TH-positive cells per DAPI in confocal images of PBS- or MPTP-treated Nlrp3 +/+ or Nlrp3£¿/£¿ mice (n£¿=£¿4). f Quantification of striatal dopamine levels of PBS- or MPTP-treated Nlrp3+/+ or Nlrp3£¿/£¿ mice as determined by HPLC-MS/MS (Nlrp3+/+ mice, PBS (n£¿=£¿11), MPTP (n£¿=£¿11); Nlrp3£¿/£¿ mice, PBS (n£¿=£¿11), MPTP (n£¿=£¿10)). Data were expressed as the mean£¿¡À£¿SEM. Asterisks indicate significant differences (*P£¿<£¿0.05, **P£¿<£¿0.01, ***P£¿<£¿0.001
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329843/