%0 Journal Article
%T Continuous, label-free, 96-well-based determination of cell migration using confluence measurement
%A Christian Mayr
%A Daniel Neureiter
%A Heidemarie Dobias
%A Julia Fuchs
%A Katharina Helm
%A Markus Ritter
%A Marlena Beyreis
%A Martin Gaisberger
%A Martin Jakab
%A Martin Pichler
%A Tobias Kiesslich
%J Archive of "Cell Adhesion & Migration".
%D 2019
%R 10.1080/19336918.2018.1526612
%X Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro
%K Migration
%K gap-closure assay
%K confluence measurement
%K non-endpoint
%K high-throughput
%K continuous measurement
%K cytochalasin D
%K ouabain
%K epidermal growth factor
%K A549 human lung carcinoma cells
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527382/