%0 Journal Article %T Cell death in ectopic calcification of the kidney %A Dorella Del Prete %A Federica Mezzabotta %A Federica Quaggio %A Franca Anglani %A Giovanna Priante %A Lisa Gianesello %A Monica Ceol %A Rosalba Cristofaro %J Archive of "Cell Death & Disease". %D 2019 %R 10.1038/s41419-019-1697-8 %X a Representative immunofluorescence images of DAPI and TUNEL-stained apoptotic nuclei of HK-2 cells grown in NH OsteoDiff medium for 1, 5, and 15 days. The images show DNA fragmentation localized in the nodule (bar£¿=£¿25£¿¦Ìm). b von Kossa staining images of cells cultured in NH OsteoDiff medium (bar£¿=£¿100£¿¦Ìm) show calcium phosphate deposition in multilayered cell nodules. c ESEM analysis. Semi-quantitative measure of the composition of the inclusions and ESEM images of selected areas of cells cultured in NH OsteoDiff medium (bar£¿=£¿500£¿¦Ìm and 10£¿¦Ìm up square) (17). d Sequence of events for the onset of nephrocalcinosis suggested by the in vitro calcification process observe %K Cell biology %K Diseases %U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565680/