%0 Journal Article
%T Vimentin activation in early apoptotic cancer cells errands survival pathways during DNA damage inducer CPT treatment in colon carcinoma model
%A Anindya Goswami
%A Anmol Kumar
%A Aparna Golani
%A Archana Katoch
%A Aviral Kumar
%A Debasis Nayak
%A Jedy Jose
%A Lekha Dinesh Kumar
%A Mir Mohd Faheem
%A Rakesh Kumar
%A Reyaz Ur Rasool
%A Souneek Chakraborty
%A Sumit G Gandhi
%A Syed Mudabir Ahmad
%A Vijay Lakshmi Jamwal
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1690-2
%X a HCT-116 and A549 cells were treated with 250£¿nM of CPT for 0, 12, 24, 36, and 48£¿h and checked for the expression of Vimentin, pser38Vimentin, Snail-1, ATM, ¦Â-catenin, and E-cadherin through western blot analysis. ¦Â-actin was used as loading control. b Immunocytochemistry was performed in HCT-116 cells treated with vehicle and CPT (250£¿nM) for 36£¿h for checking the expression of Vimentin, E-cadherin (green fluorescence). Nuclear staining was done with DAPI containing mounting media. Magnification of the images£¿=£¿¡Á63, c Analysis of the morphological features of HCT-116 cells through microscopic observation after exposure of cells to CPT for increasing time points (magnification£¿=£¿¡Á20). d Cells were treated with CPT (250£¿nM) for 24, 36, and 48£¿h along with vehicle and tested for their ability to degrade gelatin matrix and invadopodia formation through FITC-gelatin degradation assay. Blue stains indicate nuclear staining through DAPI mounting media. Images were taken at ¡Á20 magnification. Bar graph showing the threshold area of degradation quantified through Image j analysis (n£¿=£¿3, error bars£¿¡À£¿s.d.). e, f Induced AhCre-ErT Apcfl/fl mice were treated with CPT (0.4, 0.8, and 12.2£¿mg/kg) for 24 and 48£¿h and the dissected intestinal tissues were sectioned and subjected to immunohistochemistry, to analyze the expression of Vimentin and pser38Vimentin. Images were taken at ¡Á20 magnificatio
%K Metastasis
%K Chemotherapy
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6565729/