%0 Journal Article
%T Keratinocyte differentiation promotes ER stress-dependent lysosome biogenesis
%A Amitabha Majumdar
%A Mruthyunjaya Mathapathi Swamy
%A Rezwan Shariff
%A Saloni Patel
%A Sarmistha Mahanty
%A Shruthi Shirur Dakappa
%A Subba Rao Gangi Setty
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1478-4
%X a BF analysis of control and CaCl2-incubated (at ~60£¿h) cells. b CLH (in ¦Ìm) was measured (~60 cells, n£¿=£¿3) in each condition and then plotted (mean£¿¡À£¿s.e.m). c IFM analysis of LAMP-1 stained control and differentiated keratinocytes. Arrowheads point to LAMP-1 compartments and arrow indicates their distribution and increased number in differentiated cells. DL (in ¦Ìm) in each condition was measured (~60 cells, n£¿=£¿3) and indicated (mean£¿¡À£¿s.e.m.). d The cellular distribution of LAMP-1 organelles was quantified visually (~100 cells, n£¿=£¿3) and then plotted (mean£¿¡À£¿s.e.m). e¨Ch IFM analysis of keratinocytes internalized with either lysotracker or DQ-BSA, or transfected with Arl8b-GFP or GFP-Rab7, fixed and stained for LAMP-1. Arrowheads point to the LAMP-1-positive organelles. The degree of colocalization (Pearson¡¯s coefficient, r) between markers is indicated separately (mean£¿¡À£¿s.e.m., n£¿=£¿3). Nuclei are stained with Hoechst 33258 and the insets are magnified view of the white boxed areas. Scale bars, 10£¿¦Ìm. i Glucocerebrosidase (GBA) activity was normalized with cell number and then plotted (fold change in mean£¿¡À£¿s.e.m., n£¿=£¿3). j Immunoblotting analysis of keratinocytes. The fold change in protein levels is indicated. * Indicates non-specific bands detected by the antibodies. k Cell surface levels of LAMP-1 and £¿2 in control and differentiated cells. Fold change in mean fluorescence intensity (MFI) was calculated (n£¿=£¿3 in quadruplicates) and then plotted (mean£¿¡À£¿s.e.m.). *p£¿¡Ü£¿0.05 and ***p£¿¡Ü£¿0.00
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425001/