%0 Journal Article
%T Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer¡¯s disease mouse model
%A Armando A. Genazzani
%A Dmitry Lim
%A Eleonora Conte
%A Emilio Marengo
%A Federico Alessandro Ruffinatti
%A Francesca Rocchio
%A Francesca Ronco
%A Laura Tapella
%A Marcello Manfredi
%A Maria Angela Sortino
%A Mariagrazia Grilli
%A Mariangela Chisari
%A Pier Luigi Canonico
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-018-1264-8
%X a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100£¿¦Ìm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50£¿¦Ìm. The images shown in (a, b) are representative of n£¿=£¿3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean£¿¡À£¿SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In (b, c), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328590/