%0 Journal Article
%T Erythropoietin inhibits chemotherapy-induced cell death and promotes a senescence-like state in leukemia cells
%A David Miller
%A Lidia Kazakova
%A Samuel Benchimol
%A Thuc-Nghi Duc Pham
%A Weili Ma
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-018-1274-6
%X a DA3/EPOR cells were cultured in the absence of EPO for 1.5£¿h and then stimulated with EPO (1£¿U/ml) for the times indicated. JAK2 and AKT kinase activation was determined by western blot analysis using a phospho-specific Tyr 1007/Tyr 1008 antibody (pJAK2) and phospho-specific Ser 473 antibody (pAKT) respectively. b Cells were cultured in the absence of EPO for 1£¿h followed by 6£¿h of treatment with Dox (200£¿ng/ml) or DNR (0.25£¿¦ÌM), with or without EPO (1£¿U/ml). The proportion of apoptotic cells (cells with <2£¿N DNA content) was determined by flow cytometry after propidium iodide staining. Error bars represent SEM (N£¿=£¿3 biological replicates) and p values were obtained via Student¡¯s two-tailed t test. c Cells were cultured in the absence of EPO for 1.5£¿h, followed by 20£¿h treatment with Dox (200£¿ng/ml) or DNR (0.25£¿¦ÌM) in the presence or absence of EPO (1£¿U/ml). Caspase-3 activity was measured by flow cytometry. d Cells were cultured in the absence of EPO for 1.5£¿h, exposed to 6£¿Gy ¦Ã-radiation and cultured for an additional 6£¿h in the presence or absence of EPO (1£¿U/ml) before assessment of apoptosis by TUNEL assay. Error bars represent SEM (N£¿=£¿3 biological replicates
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325163/