%0 Journal Article
%T miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock
%A Chao Gu
%A Dongmei Zhou
%A Guangze Yang
%A Hongkun Liu
%A Huiyun Yang
%A Jieyou Zhang
%A Juhong Yang
%A Lijuan Zhang
%A Qi Zhang
%A Qing Xi
%A Rongxin Zhang
%A Shuguang Duo
%A Xiangdong Guo
%A Yan Li
%A Yurong Da
%A Zhenyi Xue
%A Zhi Yao
%A Zimu Zhang
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1713-z
%X a, b BMDMs were primed with 500£¿ng/ml LPS for 4£¿h and then stimulated or not with 5£¿mM ATP or 20£¿mM nigericin for 30£¿min. Lysates were immunoblotted for caspase-1, and supernatants were analyzed for IL-1¦Â. c¨Ch BMDMs were primed with 500£¿ng/ml LPS for 4£¿h and stimulated with ATP or nigericin for different lengths of time. Lysates were immunoblotted for caspase-1 (c, d), and supernatants were analyzed for IL-1¦Â (e, f) and LDH (g, h). i, j, k BMDMs were transfected with Lipo3000 (mock) or dsDNA as described in the Methods section. Lysates were immunoblotted for caspase-1 (i), and supernatants were analyzed for IL-1¦Â (j) and LDH (k). The data represent the mean£¿¡À£¿SD of one among three biological replicates, with three technical replicates each (*P£¿<£¿0.05; **P£¿<£¿0.01; Student¡¯s t test
%K Cell death and immune response
%K Bacterial infection
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6561921/