%0 Journal Article
%T MiR-210-3p protects endometriotic cells from oxidative stress-induced cell cycle arrest by targeting BARD1
%A Chao Li
%A Libing Shi
%A Lulu Liu
%A Qianmeng Huang
%A Songying Zhang
%A Wenzhi Xu
%A Xiang Lin
%A Xiaona Lin
%A Yibin Pan
%A Yinli Zhang
%A Yongdong Dai
%A Yunshan Zhu
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1395-6
%X a Immunohistochemistry photomicrographs of hypoxia-inducible factor 1¦Á (HIF-1¦Á) (the key regulator of hypoxia, which was stimulated by ROS) and 8-OHdG (an oxidative stress-induced DNA damage marker) in normal endometrium and paired eutopic and ectopic tissues. Scale bars£¿=£¿100£¿¦Ìm for ¡Á100 images. Scale bars£¿=£¿50£¿¦Ìm for ¡Á£¿400 images. b Expression of HIF-1¦Á in endometrial stromal cells (upper panel) and endometrial glandular cells (lower panel) by H-score (y-axis) was significantly higher in eutopic endometria and ectopic lesions than in normal endometria, with ectopic lesions showing the highest staining (n£¿=£¿27 for normal endometria, n£¿=£¿57 for paired eutopic and ectopic tissues). *P£¿<£¿0.05 versus normal endometrial stromal cells; #P£¿<£¿0.05 versus eutopic endometrial stromal cells. One-way ANOVA with LSD for multiple comparisons. c Expression of 8-OHdG in endometrial stromal cells (upper panel) and endometrial glandular cells (lower panel) by H-score (y-axis) was significantly higher in eutopic endometria and ectopic lesions than in normal endometria, with ectopic lesions showing the highest staining (n£¿=£¿27 for normal endometria, n£¿=£¿57 for paired eutopic and ectopic tissues). *P£¿<£¿0.05 versus normal endometrial stromal cells; #P£¿<£¿0.05 versus eutopic endometrial stromal cells. One-way ANOVA with LSD for multiple comparisons. d Representative fluorescence microscope images from alkaline comet assays of ESCs (left panel) and Ishikawa cells (right panel) after exposure to normoxia or hypoxia culture for 28 days. Scale bar£¿=£¿200£¿¦Ìm for ¡Á100 images. Scale bar£¿=£¿100£¿¦Ìm for ¡Á400 images. The tail lengths, tail DNA percentage and tail olive moments of comets were analysed using OpenComet. n£¿=£¿5 for ESC
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374490/