%0 Journal Article
%T circHECTD1 facilitates glutaminolysis to promote gastric cancer progression by targeting miR-1256 and activating ¦Â-catenin/c-Myc signaling
%A Jinguo Wang
%A Juan Cai
%A Junfeng Wang
%A Linhu Liang
%A Min Huang
%A Xianjun Chen
%A Xueliang Zuo
%A Zhengrong Zhang
%A Zhiqiang Chen
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1814-8
%X a Scatter plot showing differentially expressed circRNAs in GC tissues and paired peritumor samples. b circHECTD1 expression was examined in 50 pairs of GC tissues and peritumor samples using qRT-PCR. The expression level of circHECTD1 was significantly higher in GC tissues than in peritumor specimens. The data are shown as the mean£¿¡À£¿SEM, n£¿=£¿50. ***P£¿<£¿0.001 vs. peritumor tissues. c circHECTD1 expression was investigated in GC cell lines (BGC823, MKN45, HGC27, AGS, MGC803, and SGC7901) and the normal human gastric mucosal epithelial cell line GES-1. The data are shown as the mean£¿¡À£¿SEM, n£¿=£¿3. **P£¿<£¿0.01, ***P£¿<£¿0.001 vs. GES-1. d Sanger sequencing was used to validate the presence of circHECTD1. The arrow indicates the head-to-tail splicing sites of circHECTD1. e Representative fluorescence in situ hybridization (FISH) images showing the dominantly cytoplasmic distribution of circHECTD1. f High circHECTD1 expression levels were observed in patients with lymph node metastasis (LNM). The data are shown as the mean£¿¡À£¿SEM, n£¿=£¿50. **P£¿<£¿0.01 vs. the LNM negative group. g The expression level of circHECTD1 was higher in the AJCC stage III group than in the AJCC stage I/II group. The data are shown as the mean£¿¡À£¿SEM, n£¿=£¿50. ***P£¿<£¿0.001 vs. the AJCC stage I/II group. h A Kaplan¨CMeier curve showed that the overall survival of GC patients with high circHECTD1 levels was worse than that of patients with low circHECTD1 levels. P£¿=£¿0.01
%K Gastric cancer
%K Gastrointestinal cancer
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6675787/