%0 Journal Article
%T Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve
%A Ecem Esencan
%A Emre Seli
%A Man Zhang
%A Muhammed Burak Bener
%A Richard Scott III
%A Tamas Horvath
%A Tianren Wang
%A Zongliang Jiang
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1799-3
%X a Fertility of Mfn1£¿/£¿ and WT female mice (8-week-old) was assessed by mating with WT males of proven fertility (male/female; 1:2) for 12 weeks. The average number of delivered pups per litter per mouse was recorded. Data presented as mean£¿¡À£¿SEM. N£¿=£¿7 for each genotype; ***p£¿<£¿0.001 vs. WT using t-test. b Mfn1£¿/£¿ and WT female mice (8-week-old) were superovulated by PMSG and hCG, and the number of mature (MII) oocytes collected from oviducts was counted. Data represent mean£¿¡À£¿SEM. N£¿=£¿6 for each genotype; ***p£¿<£¿0.001 vs. WT using t-test. c and d Ovaries from Mfn1£¿/£¿ and WT mice (8-week-old) primed with PMSG (d) or unprimed (c) were fixed, embedded, sectioned, and stained with hematoxylin and eosin. Follicle counts using 3 mice ovaries for each genotype were performed. No antral follicles were found in Mfn1£¿/£¿ ovaries, with or without PMSG stimulation. e, f, g and h Representative micrographs ovarian sections from Mfn1£¿/£¿ and WT mice (8-week-old) with (f and h) or without (e and g) PMSG stimulation. i The number of secondary follicle subtypes per ovary was quantified as described in Materials and Methods section (n£¿=£¿4 mice per genotype). j The diameters of secondary follicle subtypes per ovary were measured (n£¿=£¿4 mice per genotype). k Secondary follicle-enclosed oocytes and granulosa cells were collected from Mfn1£¿/£¿ and WT ovaries. qRT-PCR was performed to determine Gdf9 and Bmp15 mRNA expression in Mfn1£¿/£¿ and WT oocytes. l qRT-PCR analysis of Activin¦ÂB, Follistatin, and kit-ligand in Mfn1£¿/£¿ and WT granulosa cells was performed. m, n PCNA immunofluorescence assays and the quantification of PCNA-positive granulosa cells in Mfn1£¿/£¿ and WT ovaries are shown. o Serum FSH levels in sera of Mfn1£¿/£¿ and WT mice (n£¿=£¿7¨C11 per genotype). Data presented as mean£¿¡À£¿SEM. *p£¿<£¿0.05, **p£¿<£¿0.01 vs. WT using t-test. T4: follicles with two layers of cuboidal granulosa cells, T5a: follicles with three layers of granulosa cells, T5b: follicles with many layers of granulosa cells but no antrum. Data represent mean£¿¡À£¿SEM. *p£¿<£¿0.05 vs. WT using t-tes
%K Cell proliferation
%K Oogenesis
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646343/