%0 Journal Article
%T Autophagosome accumulation-mediated ATP energy deprivation induced by penfluridol triggers nonapoptotic cell death of lung cancer via activating unfolded protein response
%A Chi-Li Chung
%A Guo-Zhou Cheng
%A Hsiang-Ching Huang
%A Jer-Hwa Chang
%A Michael Hsiao
%A Ming-Hsien Chien
%A Wei-Jiunn Lee
%A Wen-Yueh Hung
%A Yu Cheng
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1785-9
%X a Two NSCLC cell lines, A549 (wild-type EGFR) and HCC827 (mutant EGFR, del E746-A750), were treated with different concentrations of penfluridol for 24, 48, or 72£¿h, and cell viability was determined with an MTS assay. b A549 or HCC827 cells were treated with the vehicle or penfluridol (5£¿¦ÌM) for 24£¿h; then, the cell morphology was photographed using a phase-contrast microscope (¡Á100). c NSCLC cells and normal lung epithelial cells (BEAS-2b) were treated with the indicated concentrations of penfluridol for 24£¿h, and the half maximal inhibitory concentration (IC50) of these cells was determined by an MTS assay. d A549 and HCC827 cells were treated with the vehicle or penfluridol (1.25¨C20£¿¦ÌM) for 24£¿h; then, the death-inducing effects of penfluridol on cells were determined by counting the colonies formed. e A549 and HCC827 cells were treated with 1.25 or 2.5£¿¦ÌM of penfluridol for a transwell migration assay. Left panels of d, e: representative photomicrographs. Right panels of d, e: values are presented as the mean£¿¡À£¿SD from three independent experiments. *p£¿<£¿0.05, ***p£¿<£¿0.001 compared to the vehicle grou
%K Drug development
%K Non-small-cell lung cancer
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6629704/