%0 Journal Article
%T Establishment and Characteristics of a Novel Mantle Cell Lymphoma-derived Cell Line and a Bendamustine-resistant Subline
%A HISAO NAGOSHI
%A JUNYA KURODA
%A MASAFUMI TANIWAKI
%A SAORI MAEGAWA
%A SHIGEO HORIIKE
%A SHINSUKE MIZUTANI
%A TAKU TSUKAMOTO
%A TOMOKO TAKIMOTO-SHIMOMURA
%A TSUTOMU KOBAYASHI
%A YAYOI MATSUMURA-KIMOTO
%A YOSHIAKI CHINEN
%A YOSHIMI MIZUNO
%A YUJI SHIMURA
%A YUTO FUJIBAYASHI
%J Archive of "Cancer Genomics & Proteomics".
%D 2018
%R 10.21873/cgp.20080
%X Background/Aim: Bendamustine hydrochloride (BH) is a key therapeutic agent for mantle cell lymphoma (MCL), while the mechanism underlying BH-resistance has not been verified. Materials and Methods: We compared molecular/biological characteristics of a newly-generated MCL-derived cell line KPUM-YY1 and its BH-resistant subline KPUM-YY1R. Results: The growth-inhibitory IC50 for BH was 20 ¦ÌM in KPUM-YY1 cells, while cell proliferation was not inhibited by up to 60 ¦ÌM BH in KPUM-YY1R cells. Compared to KPUM-YY1 cells, gene expression profiling in KPUM-YY1R cells revealed up-regulation of 312 genes, including ABCB1 encoding P-glycoprotein (P-gp), and microsomal glutathione S-transferase 1 (MGST1). Addition of either a P-gp inhibitor or a GST inhibitor, at least partly, restored sensitivity to BH in KPUM-YY1R cells. In addition, KPUM-YY1R cells showed cross-resistance against various anti-MCL chemotherapeutics. Conclusion: BH resistance is mediated by overlapping mechanisms with overexpression of ABCB1 and MGST1, and is potentially accompanied by multidrug resistance in MCL
%K Mantle cell lymphoma
%K bendamustine
%K resistance
%K ABCB1
%K MGST1
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971014/