%0 Journal Article
%T Cardioprotective role of APIP in myocardial infarction through ADORA2B
%A Bitna Lim
%A Changwon Kho
%A Han-Woong Lee
%A Jae Gyun Oh
%A Jae-il Roh
%A Jang-Whan Bae
%A Kwangmin Jung
%A Se-Hoon Hong
%A Woo-Jin Park
%A Yong-Keun Jung
%A Youngdae Gwon
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1746-3
%X a每c Increase of both Apip and Adora2b transcripts in cardiac tissue from patients with heart failure. Apip (a) and Adora2b (b) transcripts in cardiac tissue from patients with heart failure or controls were quantified by real-time RT-PCR analysis (Control group: nˋ=ˋ29, Heart failure patient group: nˋ=ˋ18). Statistical significance was determined by Student＊s t test. Levels of APIP and ADORA2B transcripts were plotted with mean Pearson correlation coefficient (0.912) (c). d APIP regulates ADORA2B in mice. Representative western blot showing ADORA2B protein levels in the heart of WT, APIPTg/Tg, APIPTg/+, and Apip+/ˋ mice at 4每5 months of age. e每g Increase of both Apip and Adora2b transcripts, and APIP protein in the hypoxic condition. Cultured primary cardiomyocytes were incubated with normoxic (Nor., 21% O2) or hypoxic (Hypo., 1% O2) condition for 6ˋh and total RNAs were analyzed by real-time RT-PCR analysis (**Pˋ<ˋ0.01, *Pˋ<ˋ0.05, two-tailed t test) (e). Cell lysates were analyzed by western blotting (f) and the signals on the blots were quantified by densitometric analysis (**Pˋ<ˋ0.01, *Pˋ<ˋ0.05, two-tailed t test) (g). h, i Reduction of infarct area in the heart of APIPTg/+ mice. APIPTg/+ mice or WT littermate were subjected to ligation of the left descending anterior coronary artery for 24ˋh and the hearts were then stained with Evans blue and 2,3,5-triphenyltetrazolium chloride (TTC). The Evans blue-perfused area, which is not at risk, was stained blue, viable myocardium was stained red, and infarcted myocardium appeared pale. The representative images are shown (h). AAR/LV indicates percentage of LV at risk, IA/AAR, infarcted area as percentage of risk area (Male, 12 weeks, nˋ=ˋ8 mice per group, *Pˋ<ˋ0.05, N.S., not significant (Pˋ>ˋ0.05), one-way ANOVA/Bonferroni) (i). j每m Restoration of cardiac function in APIPTg/+ mice. APIPTg/+ mice and WT littermates are subjected to ligation of the left descending anterior coronary artery. Left ventricular (LV) chamber dimensions and systolic function were measured at 3 weeks after the ligation surgery. Typical echocardiographic M-mode images are shown (j). Data are represented as the meanˋ㊣ˋS.D. (male, 13每14 weeks, nˋ=ˋ3 mice per group, **Pˋ<ˋ0.01, one-tailed t test). Parameters for cardiac function: FS fractional shortening, EF ejection fraction, LVIDs LV internal diameter at systole (k每m
%K Apoptosis
%K Circulation
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602929/