%0 Journal Article
%T Expression of UPR effector proteins ATF6 and XBP1 reduce colorectal cancer cell proliferation and stemness by activating PERK signaling
%A Bartolomeus J. Meijer
%A Claudia N. Spaan
%A Gijs R. van den Brink
%A Jarom Heijmans
%A Jooske F. van Lidth de Jeude
%A Vanesa Muncan
%A Wouter L. Smit
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1729-4
%X a Quantitative RT-PCR analysis for XBP1(s) and downstream target genes upon induction of XBP1(s) expression. b Protein level of XBP1(s) and GRP78 in LS174T XBP1(s)Tet On cells. Cells were incubated for 0, 1 or 3£¿h with thapsigargin 200£¿nM. Note that transgenic XBP1(s) had a slightly lower molecular weight than endogenous XBP1(s). c Immunostaining of XBP1 in LS174T XBP1(s)Tet On cells. d Quantitative RT-PCR analysis for ATF61¨C373 and downstream target genes upon induction of ATF61¨C373 expression. e Protein level of GRP78 in LS174T ATF61¨C373 Tet On cells. Cells were treated with vehicle or doxycycline 1£¿¦Ìg/mL for 18£¿h. All data are shown as means£¿¡À£¿SEM. *P£¿<£¿0.05, **P£¿<£¿0.01, ***P£¿<£¿0.00
%K Colon cancer
%K Endoplasmic reticulum
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588629/