%0 Journal Article
%T Docosahexaenoic acid inhibits both NLRP3 inflammasome assembly and JNK-mediated mature IL-1β secretion in 5-fluorouracil-treated MDSC: implication in cancer treatment
%A Adélie Dumont
%A Aurélie Fluckiger
%A Aziz Hichami
%A Charles Thomas
%A Charlotte de Rosny
%A Cédric Rébé
%A Fran？ois Ghiringhelli
%A Hélène Berger
%A Jean-Paul Pais de Barros
%A Laurent Pichon
%A Micka？l Rialland
%A Sabrina Perrey
%A Tania Muller
%A Trinh-Le-Vi Kieu
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1723-x
%X a MSC-2 were treated by 5-FU (1？μM) for 24？h ±DHA (20 to 60？μM) and cell culture media were collected for analysis of IL-1β concentration by ELISA. Error bars represent mean？±？SD from four independent experiments. b Analysis of IL-1β secretion by ELISA in MSC-2 treated with 5-FU (1？μM) for 24？h ±DHA, eicosapentaenoic (EPA), oleic (OA), and linoleic (LA) acids at 60？μM. Error bars represent mean？±？SD from 3 to 6 independent experiments. c Analysis of IL-1β mRNA expression, by RT-qPCR, in MSC-2 treated for 12 and 24？h with 5-FU ±DHA (60？μM). Error bars represent mean？±？SD from three independent experiments. d Expression of IL-1β mRNA, by RT-qPCR, in MSC-2 treated for 12？h with 5-FU ±Lipopolysaccharide (LPS). Error bars represent mean？±？SD from three independent experiments. e Determination of plasma IL-1β content in EL4 tumor-bearing mice fed control (ctrl) or DHA-enriched diet, initiated 7 days prior to a single intraperitoneal injection of 5-FU (50？mg/kg). Plasma was collected 48？h after the injection of 5-FU. f Monitoring of tumor growth in mice fed a ctrl diet (n？=？8, black) or DHA-enriched diet (n？=？9, purple) and in mice treated with a single 5-FU injection (50？mg/kg; blue arrow) and fed a ctrl diet (n？=？9, gray) or DHA-enriched diet (n？=？9, red). Error bars represent mean？±？SEM. *p？<？0.05; **p？<？0.01; ***p？<？0.001; ****p？<？0.0001; ns nonsignifican
%K Cancer
%K Interleukins
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584690/