%0 Journal Article
%T Exosomes derived from M1 macrophages aggravate neointimal hyperplasia following carotid artery injuries in mice through miR-222/CDKN1B/CDKN1C pathway
%A Aijun Sun
%A Hong Zhu
%A Hongtao Shi
%A Huan Zhao
%A Junbo Ge
%A Kai Hu
%A Rifeng Gao
%A Rongle Liu
%A Xiao Li
%A Xinyu Weng
%A Yunzeng Zou
%A Zeng Wang
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1667-1
%X a, b M1 macrophages were positively associated with HA-VSMC proliferation. GW4869, a well-known inhibitor of exosome secretion, is used at a concentration of 10£¿¦ÌM to reduce the release of exosome from M1M. *P£¿<£¿0.05, versus Control group; £¿P£¿<£¿0.05, versus co-culture group. n£¿=£¿3, each group. c Schematic co-culture model for M1M affect VSMCs proliferation. d, e M1M were positively associated with HA-VSMC migration. GW4869, a well-known inhibitor of exosome secretion, is used at a concentration of 10£¿¦ÌM to reduce the release of exosome from M1M. *P£¿<£¿0.05, versus Control group; £¿P£¿<£¿0.05, versus co-culture group. n£¿=£¿3, each group. f Schematic co-culture model for M1M affect VSMCs migration. g The ultrastructure of R1-EXO and T1-EXO showed typical cup-shaped morphology by transmission electron microscopy. h NTA demonstrates the size distribution of T1-EXO and R1-EXO revealing a size peak of 123 and 132£¿nm, respectively. i, j The expression of exosomes markers, Alix, Hsp70, and CD63 were confirmed by immunoblotting. A total of 20£¿¦Ìg protein from cell lysis and 20£¿¦Ìg protein from exosomes lysis was loaded into each lane. *P£¿<£¿0.05, versus Control group. n£¿=£¿3, each group. All data were expressed as mean£¿¡À£¿SEM from three individual experiment
%K Cell invasion
%K Carotid artery disease
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541659/