%0 Journal Article
%T Basal p53 expression is indispensable for mesenchymal stem cell integrity
%A Christopher L. Haga
%A Cori N. Booker
%A Donald G. Phinney
%A Jacqueline Strivelli
%A Siddaraju V. Boregowda
%A Veena Krishnappa
%J Archive of "Cell Death and Differentiation".
%D 2018
%R 10.1038/s41418-017-0004-4
%X Basal p53 expression maintains MSC integrity. a Quantification of stimulus-induced adipogenic (left panel) and osteogenic (right panel) differentiation of p53+/+ vs. p53£¿/£¿ MSCs. Data are mean£¿¡À£¿S.D. of experiments (n£¿=£¿4) repeated twice. **p£¿<£¿0.01, ***p£¿<£¿0.005 by Student¡¯s t-test. b CFU-F activity of p53+/+ vs. p53£¿/£¿ MSCs. Data are mean£¿¡À£¿S.D. of experiments (n£¿=£¿6) repeated twice. ***p£¿<£¿0.005 by Student¡¯s t-test. c Flow cytometric analysis of PI-stained cells showing percentage of p53+/+ vs. p53£¿/£¿ MSCs in G1, S, and G2 phases of cell cycle. d CAFC activity of p53+/+ vs. p53£¿/£¿ MSCs. Data are mean£¿¡À£¿S.D. of experiments (n£¿=£¿6) repeated twice. ***p£¿<£¿0.005 by Student¡¯s t-test. e Methylcellulose colony-forming assays using conditioned media from p53+/+ vs. p53£¿/£¿ MSCs. Data are mean£¿¡À£¿S.D. of experiments (n£¿=£¿3) repeated twice. *p£¿<£¿0.05, **p£¿<£¿0.01, ***p£¿<£¿0.005 by Student¡¯s t-test. f qPCR analyses of the indicated mRNAs in p53+/+ vs. p53£¿/£¿ MSCs. All cytokine levels were significantly (**p£¿<£¿0.01) different between cell types by Student¡¯s t-test (n£¿=£¿3) except where indicated. g Luminex-based analyses of conditioned media collected from monolayer cultures of p53+/+ vs. p53£¿/£¿ MSCs. ***p£¿<£¿0.005 by Student¡¯s t-test. h ChIP showing pull-down of Kitl promoter sequences using a RNA Pol II (positive control) or p53 antibody from cell extracts of p53+/+ MSCs treated with Nutlin-3a (20£¿¦ÌM). **p£¿<£¿0.01 compared to IgG control by ANOVA and Tukey post hoc test. i p53 mRNA and protein expression quantified by qPCR (upper panel) and immunoblot analysis (bottom panel), respectively, in p53+/+ MSCs transfected with a scrambled (Scr) and p53-specific siRNA. qPCR data are mean£¿¡À£¿SD of experiments (n£¿=£¿3) repeated twice. ***p£¿<£¿0.005 by Student¡¯s t-test. j, k Effect of siRNAs on CFU-F (j) and CAFC (k) activity in p53+/+ MSCs. Data are mean£¿¡À£¿SD of experiments (n£¿=£¿6) repeated twice. ***p£¿<£¿0.005 by Student¡¯s t-test. l Quantification of stimulus-induced adipogenic (left panel) and osteogenic (right panel) differentiation of siRNA-transfected p53+/+ MSCs. Data are mean£¿¡À£¿SD (n£¿=£¿4). **p£¿<£¿0.01 compared to unstimulated control and ++p£¿<£¿0.01 compared to scrambled (Scr) by ANOVA and Tukey post hoc tes
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5883824/