%0 Journal Article
%T DNA damage and apoptosis induced by a potent orally podophyllotoxin derivative in breast cancer
%A Dan Zhang
%A Fangfang Li
%A Gang Zhang
%A Hua Sun
%A Ning Wei
%A Shaoyu Wu
%A Xiuqi Bao
%A Yajie Wang
%A Yuanchao Gao
%A Zhiyan Xiao
%J Archive of "Cell Communication and Signaling : CCS".
%D 2018
%R 10.1186/s12964-018-0263-9
%X Effect of XWL-1-48 on TopoII activity and growth of breast cancer cells. a The chemical structure of podophylloloxin, GL331, Etoposide (VP16), Teniposide and XWL-1-48 is shown; b Superimposition of Top2¦Á homology model (cyan) and Top2¦Â crystal structure (Green), shown in cartoon representation. XWL-1-48 pose from the Top2¦Â crystal structure (stick representation, carbons yellow) shown for clarity; c Molecular docking study of XWL-1-48 with carbons in cyan to crystal structure of Top2¦Á-DNA homology model. Only interacting residues were labeled. Hydrogen bonds are shown as purple dotted lines; d To determine the effect of XWL-1-48 on TopoII activity, kDNA decatenation assay was performed as described in materials and methods; e The cytotoxic effect of XWL-1-48 on growth of MCF-7 and MDA-MB-231 cells was determined by MTT assay. IC50 was calculated by Prism6.0 software; f MCF-7 or MDA-MB-231 cells were seeded in a 6-well plate, and incubated with XWL-1-48 (1, 2.5, 5 nM) or GL331 (5 nM) for 24 h. After 10¨C14 days, colonies (>£¿50 cells) were fixed and manually counted. A representative of three experiments is shown. Data were shown as mean£¿¡À£¿SD of three independent experiments. *p£¿<£¿0.05, **p£¿<£¿0.01 vs. contro
%K Breast cancer
%K TopoisomeraseII
%K DNA damage
%K p53
%K Mdm2
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122736/