%0 Journal Article
%T lncRNA KRAL reverses 5-fluorouracil resistance in hepatocellular carcinoma cells by acting as a ceRNA against miR-141
%A Chenwei Pan
%A Jianjian Zheng
%A Liang Shi
%A Lili Wu
%A Xiangyang Lin
%A Xin Wei
%A Yifen Shi
%J Archive of "Cell Communication and Signaling : CCS".
%D 2018
%R 10.1186/s12964-018-0260-z
%X Downregulation of KRAL is associated with 5-fluorouracil resistance in HCC cells. a The IC50 values of 5-FU in HepG2, SMMC-7721, HepG2/5-FU, and SMMC-7721/5-FU cells. Data are expressed as the mean£¿¡À£¿SD; columns: mean of three independent experiments; *p£¿<£¿0.05, **p£¿<£¿0.01. b The protein levels of Keap1 in HepG2, SMMC-7721, HepG2/5-FU, and SMMC-7721/5-FU cells were detected by western blot. GAPDH was used as a reference, data are expressed as the mean£¿¡À£¿SD; the bar graph indicates the normalized values from at least 3 separate experiments; *p£¿<£¿0.05, #p£¿<£¿0.05 vs the respective parent cells. c qRT-PCR was performed to detect the relative expression of KRAL in HepG2 and SMMC-7721 cells treated with different non-cytotoxic doses of 5-FU, GAPDH was used as a reference, Data are expressed as the mean£¿¡À£¿SD; columns: normalized mean values of three independent experiments; *p£¿<£¿0.05, #p£¿<£¿0.05, &p£¿<£¿0.05. d The protein level of Keap1 in HepG2 and SMMC-7721 cells treated with different non-cytotoxic concentrations of 5-FU was analysed by western blot. GAPDH was used as a reference; data are expressed as the mean£¿¡À£¿SD; the bar graph indicates the normalized values from at least 3 separate experiments; *p£¿<£¿0.05 vs the 0 ¦Ìg/mL group, #p£¿<£¿0.05 vs the 0.05 ¦Ìg/mL or 0.15 ¦Ìg/mL group, &p£¿<£¿0.05 vs the 0.1 ¦Ìg/mL or 0.3 ¦Ìg/mL grou
%K Long non-coding RNA (lncRNA)
%K miR-141
%K Keap1
%K 5-fluorouracil
%K Chemoresistance
%K Hepatocellular carcinoma
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098660/