%0 Journal Article
%T Tissue-engineered smooth muscle cell and endothelial progenitor cell bi-level cell sheets prevent progression of cardiac dysfunction, microvascular dysfunction, and interstitial fibrosis in a rodent model of type 1 diabetes-induced cardiomyopathy
%A Amanda N. Steele
%A Anahita Eskandari
%A Andrew B. Goldstone
%A Arnar B. Ingason
%A Bryan B. Edwards
%A Hanjay Wang
%A Kevin J. Jaatinen
%A Lyndsay M. Stapleton
%A Masashi Kawamura
%A Michael J. Paulsen
%A Shigeru Miyagawa
%A Vi N. Truong
%A Y. Joseph Woo
%A Yasuhiro Shudo
%A Yoshiki Sawa
%J Archive of "Cardiovascular Diabetology".
%D 2017
%R 10.1186/s12933-017-0625-4
%X Characterization of SMCs, EPCs, and EPC-SMC bi-level cell sheets. a SMC-EPC bi-level cell sheet manufacturing protocol. b¨Cg Immunocytochemistry demonstrated ¦ÁSMA and SM22¦Á on SMCs (b, c), and CD31, CD34, vWF, and VEGF-R2 on EPCs (d¨Cg). Images of isotype controls of mouse, rabbit, and goat IgG were provided (h¨Cj). Scale bar = 50 ¦Ìm. k Percentages of each antigen for SMCs and EPCs were high, and demonstrated that our protocol yielded SMCs and EPCs with high purity. l A round-shaped scaffold-free SMC-EPC bi-level cell sheet in a 35 mm-dish. m Immunostaining of the SMC-EPC bi-level cell sheet with anti-vWF (green) and anti-¦ÁSMA (red) antibodies. The cell nuclei were counterstained with DAPI (blue). Scale bar = 50 ¦Ìm. EPC endothelial progenitor cell, SMC smooth muscle cell, MSC mesenchymal stem cell, DAPI 4¡ä,6-diamidino-2-phenylindole, ¦ÁSMA ¦Á smooth muscle actin, sm22¦Á smooth muscle protein 22-¦Á, vWF von Willebrand factor, VEGF-R2 vascular endothelial growth factor-receptor 2, ms IgG mouse immunoglobulin G, rb IgG rabbit immunoglobulin G, gt IgG gout immunoglobulin
%K Diabetic cardiomyopathy
%K Tissue engineering
%K Cell therapy
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668999/