%0 Journal Article
%T Abrogation of store-operated Ca2+ entry protects against crystal-induced ER stress in human proximal tubular cells
%A Bidhan C. Bandyopadhyay
%A Farai C. Gombedza
%A Samuel Shin
%A Yianni L. Kanaras
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0203-5
%X Control (noncrystal) CaP, CaOx, and CaP£¿+£¿CaOx (mixed) crystals were introduced into HK2 cells for 24£¿h. Crystal internalization effects on [Ca2+]i mobilization in GPCR-activated HK2 cells were measured by Ca2+ imaging. Mean fluorescent traces of Fura-2 loaded HK2 cells bathed in Ca2+-free solution and then 2.0£¿mM Ca2+ and neomycin CaSR activation were obtained for a Control; b CaP; c CaOx; and d Mixed crystals. Representative bar diagrams depict (e) peak [Ca2+]i release; f [Ca2+]i rise; and g percent reduction Ca2+ entry. Two-tailed t-test was used for statistical comparison in (e¨Cg). Statistically significant differences are indicated (mean£¿¡À£¿SEM) from four different experiments. Levels of significance are indicated as *p£¿<£¿0.05; **p£¿<£¿0.01 as shown in the bar diagram
%K Calcium signalling
%K Mechanisms of disease
%K Stress signalling
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680047/