%0 Journal Article
%T Platelet factor 4-containing immune complexes induce platelet activation followed by calpain-dependent platelet death
%A Amina G. Daminova
%A Anastasia A. Ponomareva
%A Elmira R. Mordakhanova
%A Giang Le Minh
%A Izabella A. Andrianova
%A John W. Weisel
%A Lubica Rauova
%A Rustem I. Litvinov
%A Tatiana A. Nevzorova
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0188-0
%X Platelets were incubated for 15£¿min (light bars) and 60£¿min (dark bars) under the following conditions: untreated platelets (negative control), platelets treated with the KKO/PF4 and RTO/PF4 complexes, KKO, RTO, and PF4 alone as well as Ca2+-ionophore A23187 (positive control). Final concentrations: 10£¿¦Ìg/ml PF4, 50£¿¦Ìg/ml KKO, and RTO, 10£¿¦ÌM Ca2+-ionophore. a Annexin V-positive platelets as a fraction of all gated platelets taken as 100%: untreated platelets (n£¿=£¿17/13 for 15£¿min/60£¿min, respectively), KKO/PF4 (n£¿=£¿16/13), RTO/PF4 (n£¿=£¿3/3), KKO (n£¿=£¿16/13), RTO (n£¿=£¿3/3), PF4 (n£¿=£¿16/13), and A23187 (n£¿=£¿14/12). b CD62P-positive platelets as a fraction of all gated platelets taken as 100%: untreated platelets (n£¿=£¿8/7), KKO/PF4 (n£¿=£¿8/7), RTO/PF4 (n£¿=£¿3/3), KKO (n£¿=£¿8/7), RTO (n£¿=£¿3/3), PF4 (n£¿=£¿8/7), and A23187 (n£¿=£¿8/7). c Number of CD41- and Annexin V-double-positive microvesicles normalized by the total number of gated platelets in the corresponding sample: untreated platelets (n£¿=£¿8/9), KKO/PF4 (n£¿=£¿8/9), RTO/PF4 (n£¿=£¿3/4), KKO (n£¿=£¿8/9), RTO (n£¿=£¿3/4), PF4 (n£¿=£¿8/9), and A23187 (n£¿=£¿8/9). ¡°n¡± is the number of experiments with platelets from independent donors. *P£¿<£¿0.05 compared to a corresponding negative control. The differences between 15 and 60£¿min are insignifican
%K Mechanisms of disease
%K Autoimmunity
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591288/