%0 Journal Article
%T MEK5/ERK5 activation regulates colon cancer stem-like cell properties
%A Cec¨ªlia M. P. Rodrigues
%A Diane M. Pereira
%A Pedro M. Borralho
%A Sofia. E. Gomes
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0150-1
%X a HCT116, HT29, SW480, and SW620 cells were cultured under sphere-forming or adherent conditions. MEK5 and ERK5 phosphorylation levels were evaluated by western blot. b¨Cd SW480 adherent cultures were transfected with CA-MEK5 expression construct or empty vector. b MEK5 overexpression and ERK5 activation status were confirmed by immunoblotting. c mRNA levels of several stemness- and differentiation-associated markers were determined by qRT-PCR. d Steady-state protein levels of NANOG, OCT4, and SOX2 were evaluated by immunoblot analysis. Blots are representative of three independent experiments with similar results. Results are expressed as mean£¿¡À£¿standard error of mean from at least three independent experiments. *p£¿<£¿0.05, **p£¿<£¿0.01, and ***p£¿<£¿0.001 from respective adherent cultures (a) or empty vector cells (c). Ad Adherent, Ts tumorsphere, CA constitutively activ
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370793/