%0 Journal Article
%T The pro-apoptotic Bcl-2 family member Harakiri (HRK) induces cell death in glioblastoma multiforme
%A Ahmet Cingoz
%A Alisan Kayabolen
%A Ezgi Kaya-Aksoy
%A Fidan Seker
%A Filiz Senbabaoglu
%A Gizem Nur Sahin
%A Ilknur Sur-Erdem
%A Sercin Karahuseyinoglu
%A Tolga Lokumcu
%A Tugba Bagci-Onder
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0144-z
%X a Hrk is differentially expressed in four different established cell lines (A172, LN18, U87MG, U373). Values are normalized to the level of housekeeping gene, GAPDH. b Western blot analysis of endogenous HRK expression in A172, LN18, U87MG, U373 cell lines. c Western blot analysis of HRK in the whole cell lysate of GFP- and HRK-overexpressing GBM cell lines. d, e HRK overexpression decreases cell viability (d) and increases the activation of caspase 3/7 (e). (* denotes p£¿<£¿0.05, n.s. denotes non-significant (p£¿>£¿0.05), t-test). f Western blot analysis of PARP cleavage in GFP and HRK-overexpressing GBM cells. g Representative figures showing HRK-induced cell death in GBM cell lines, scale bars:100£¿¦ÌM. h Growth of GFP or HRK-expressing cells in long-term. t0 denotes the time of seeding, t1 denotes the time of viral transduction, t2 denotes the time of medium change. i, j General caspase inhibitor treatment (20£¿¦ÌM ZVAD-FmK or ZVA-FmK) recovers the HRK-induced changes in cell viability (i) and caspase activity (j). k TUNEL staining of GBM cell lines transduced with HRK or control vectors (green: TUNEL, blue: DAPI, scale bars: 10£¿¦ÌM
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368544/