%0 Journal Article
%T Resveratrol enhances pluripotency of mouse embryonic stem cells by activating AMPK/Ulk1 pathway
%A Aleksandra R. Knyazeva
%A Alexey V. Petukhov
%A Irina I. Suvorova
%A Nicolay D. Aksenov
%A Valery A. Pospelov
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0137-y
%X a Fluorescence-activated cell sorting (FACS) analysis of cell cycle phase distribution of mESCs untreated and treated with 10£¿¦ÌM of resveratrol for 1 and 3 days. b Top panel: cell index curves of untreated and resveratrol-treated (10£¿¦ÌM) mESCs were generated using the xCELLigence RTCA DP system. The proliferation rates were registered every 15£¿min for 55£¿h; error bars correspond as means ¡À£¿SEM (n£¿=£¿4). Bottom panel: the values were obtained at the whole proliferation curve at 0¨C55£¿h. c Western blot of total lysates from control mESCs and mESCs treated with resveratrol (10£¿¦ÌM) for the indicated periods of time; antibodies against H2AX (Ser139) and ¦Á-Tubulin for endogenous control were used. The representative of experiments repeated at least three times is shown. d Left panel: flow cytometric analysis of apoptotic cells using Annexin V surface staining and propidium iodide (PI). Shown are dot plots of the fluorescein isothiocyanate-conjugated Annexin V versus PI staining for mESCs untreated and treated with resveratrol for the indicated periods of time. Right panel: representation of flow cytometry results from the left panel as diagram; error bars correspond to the SEM calculated for three replicate
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361884/