%0 Journal Article
%T Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood
%A Alexey V. Krat
%A Ana Gargaun
%A Anna S. Kichkailo
%A Dmitry V. Veprintsev
%A Galina S. Zamay
%A Irina I. Garanzha
%A Ivan N. Lapin
%A Maxim V. Berezovski
%A Olga S. Kolovskaya
%A Tatiana I. Ivanchenko
%A Tatiana N. Zamay
%A Valentina L. Grigorieva
%A Valery A. Svetlichnyi
%A Yury E. Glazyrin
%J Archive of "Cancers".
%D 2019
%R 10.3390/cancers11030351
%X We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients
%K aptamers
%K SELEX (Systematic evolution of ligands by exponential enrichment)
%K EpCAM
%K non-small-cell lung cancer
%K circulating tumor cells
%K blood
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468627/