%0 Journal Article
%T Hyperglycemia exacerbates acetaminophen-induced acute liver injury by promoting liver-resident macrophage proinflammatory response via AMPK/PI3K/AKT-mediated oxidative stress
%A Chenyu Shi
%A Gefenqiang Shen
%A Haoming Zhou
%A Jiannan Qiu
%A Ling Lu
%A Qi Wang
%A Shun Zhou
%A Song Wei
%A Xiaojie Gan
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0198-y
%X Hyperglycemic (streptozotocin, STZ) and control (CON) mice were prepared as described in ¡°Materials and methods¡± section. APAP-induced acute liver injury or a sham procedure was performed. a Blood glucose levels were measured in both groups (n£¿=£¿6/group). b, c Liver injury was evaluated 24£¿h post APAP treatment in terms of serum AST and ALT levels (n£¿=£¿6/group). d Liver injury was evaluated in terms of liver histopathology. Representative of six mice/group. e TUNEL staining of liver sections (original magnification ¡Á200). DAPI was used for nuclear staining. Representative of six mice/group. f The ratio of TUNEL-positive cells in different experimental groups (n£¿=£¿6/group). g Bcl-2, Bcl-xL, and ¦Â-actin protein levels were measured by western blot. Representative of three experiments (mean£¿¡À£¿SD, *p£¿<£¿0.05
%K Acute inflammation
%K Kupffer cells
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6642179/