%0 Journal Article
%T Protoporphyrin IX is a dual inhibitor of p53/MDM2 and p53/MDM4 interactions and induces apoptosis in B-cell chronic lymphocytic leukemia cells
%A Joanna Zawacka-Pankau
%A Liren Jiang
%A Natasha Malik
%A Pilar Acedo
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0157-7
%X a F2H£¿-analysis of disruption of interactions of p53/MDM2 and p53/MDM4 by PpIX and nutlin in U2OS cells, when MDM2 or MDM4 is tethered in the nucleus. U2OS cells were co-transfected with LacI-GFP-MDM2 or MDM4 and RFP-p53 for 8£¿h and then treated with PpIX or nutlin for 16£¿h. Control interaction values are normalized to 100% and are shown as a red line. Averaged interaction values for the treated cells are plotted for p53/MDM2 interaction (left, green bars) and p53/MDM4 interaction (right, blue bars). Data represented as mean£¿¡À£¿s.e.m., n£¿=£¿5¨C6, *p£¿<£¿0.05, **p£¿<£¿0.01, ***p£¿<£¿0.001, Student¡¯s t-test. b Rescue of the p53 transcription activity in the presence of MDM4 by PpIX but not nutlin as assessed in the yeast-based reporter assay. The average light units relative to the transactivation activity of p53 alone and the standard errors of at least five biological repeats are presented. The Student¡¯s t-test was performed for statistical analysis with p£¿¡Ü£¿0.05. c PpIX (1£¿¦Ìg/ml) disrupts p53/MDM4 interactions in HCT 116 colon cancer cells as assessed by immunoprecipitation. The data is a representative of two independent experiments. Cropped line represents the site were the membranes were cut. X-sample not relevant to this stud
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412042/