%0 Journal Article
%T The biochemical and electrophysiological profiles of amniotic fluid-derived stem cells following Wnt signaling modulation cardiac differentiation
%A Chi-Ting Su
%A Ping-Yen Liu
%A Sheng-Nan Wu
%A Shiaw-Min Hwang
%A Yen-Wen Liu
%A Yi-Hsein Fang
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0143-0
%X a Representative images showed the appearance of undifferentiated and differentiated AFSCs, human embryonic stem cell (hESC) and hESC-derived cardiomyocytes (hESC-CMs). Undifferentiated AFSCs exhibited a heterogeneous morphology with a preponderance of fibroblastoid, mesenchymal-like cell shapes. After 14 days of differentiation, the morphology of AFSCs exhibited a rod-like appearance, different from that of human embryonic stem cell-derived cardiomyocytes. Scale bar, 200£¿¦Ìm. b Undifferentiated AFSCs and human embryonic stem cells (hESCs) expressed the pluripotent stem cell markers Nanog, Oct3/4, and SSEA4. At cardiac differentiation day 14, the expression of these 3 pluripotent stem cell markers significantly reduced in both differentiated AFSCs and hESC¨Cderived cardiomyocytes (hESC-CMs
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349909/