%0 Journal Article
%T The prodomain of caspase-3 regulates its own removal and caspase activation
%A Katelyn G. Ponder
%A Lawrence H. Boise
%J Archive of "Cell Death Discovery".
%D 2019
%R 10.1038/s41420-019-0142-1
%X a Protein expression of caspase-3 and loading control actin of parental MEFs and stable cell lines expressing empty vector pBabe, full-length caspase-3 and two catalytically inactive forms of caspase-3, C163A and C163S. b Caspase-3 activity was measured using a caspase activity assay. Cells were grown in full serum as a control or serum starved for 24£¿h. A DEVD-chromphore substrate (DEVD-p-NA) was added to the protein lysis, and light emittance was measured at 405£¿nm. c Cells were serum starved for 0, 24, and 48£¿h to induce apoptosis. Apoptosis was determined by positive annexin V/propidium iodide (PI) and analyzed for cell death using flow cytometry. Data are presented as mean£¿¡À £¿SEM of at least three independent experiments. *p£¿>£¿0.05, **p£¿>£¿0.01, ***p£¿>£¿0.00
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6349851/