%0 Journal Article
%T Repurposing CRISPR-Cas12b for mammalian genome engineering
%A Fei Teng
%A Guihai Feng
%A Jing Li
%A Kai Xu
%A Lu Guo
%A Qi Zhou
%A Qingqin Gao
%A Tianda Li
%A Tongtong Cui
%A Wei Li
%J Archive of "Cell Discovery".
%D 2018
%R 10.1038/s41421-018-0069-3
%X a Schematic illustration of the genomic architecture of CRISPR-Cas12b from Alicyclobacillus acidiphilus (NBRC 100859) (left) and the crRNA/tracrRNA duplex (right). b In vitro cleavage activity of AaCas12b at various temperatures. The cleavage rate is shown under the cleaved lanes. c In vitro validation of the PAM requirements of AaCas12b showing that PAMs matching the 5กไ-TTN sequence can be efficiently cleaved. The cleavage rate is shown under the cleaved lanes. d Cleavage site determination of AaCas12b by sequencing the cleavage products. The cleavage sites are indicated by red triangles in the left panel. TS target strand, NTS non-target stran
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6255809/