%0 Journal Article
%T HCV-associated exosomes promote myeloid-derived suppressor cell expansion via inhibiting miR-124 to regulate T follicular cell differentiation and function
%A Dechao Cao
%A Jonathan P. Moorman
%A Juan Zhao
%A Lam Nhat Nguyen
%A Lin Wang
%A Ling Wang
%A Mohamed El Gazzar
%A Qian Xie
%A Shunbin Ning
%A Xiao Y. Wu
%A Xindi Dang
%A Yingjie Ji
%A Zheng D. Morrison
%A Zhi Q. Yao
%J Archive of "Cell Discovery".
%D 2018
%R 10.1038/s41421-018-0052-z
%X a Representative dot plots analysis of TFR and TFH cells by flow cytometry. PBMCs were first gated on lymphocytes, and then T follicular cells (CD4+CXCR5+), followed by gating the Foxp3 marker to differentiate TFR (CD4+CXCR5+Foxp3+) and TFH (CD4+CXCR5+Foxp3£¿) cells, respectively. The values in the quadrants indicate the percentages of the related subsets. b Summary data for the percentage of T follicular cell (CD4+CXCR5+) frequencies in HS (n£¿=£¿12) versus chronically HCV-infected patients (n£¿=£¿16), in both unstimulated and TCR-stimulated conditions. c Summary data for the percentage of Foxp3+ cells in CD4+CXCR5+ cells in HS (n£¿=£¿12) versus HCV-infected patients (n£¿=£¿16) versus DAA-treated HCV subjects with SVR (n£¿=£¿8) in freshly isolated and TCR-stimulated PBMCs. d Summary data for the percentage of Foxp3£¿ cells in CD4+CXCR5+ cells in HS versus HCV-infected patients versus DAA-treated subjects with SVR in freshly isolated and TCR-stimulated PBMCs. e The ratio of TFR/TFH cells in HS versus HCV versus SVR in freshly isolated and TCR-stimulated PBMCs. f Representative dot plots and summary data for IL-21 expression in CD4+CXCR5+ T follicular cells. g Pooled data of IL-10 expression in CD4+CXCR5+ T follicular cells from HCV patients (n£¿=£¿13) normalized by HS (n£¿=£¿12). Each symbol represents an individual subject, and the horizontal bars represent median with interquartile. P value with significant difference is shown in each panel; NS no significanc
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131392/