%0 Journal Article
%T Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry
%A Adolfo Garc¨ªa-Sastre
%A Alexey V. Terskikh
%A Anil Mathew Tharappel
%A Anton Simeonov
%A Antonella Pinto
%A Billy Lu
%A Carles Mart¨ªnez-Romero
%A Chen Farhy
%A Chun-Teng Huang
%A Dushyantha T. Jayaweera
%A Emily M Lee
%A Guang Song
%A Guoli Ming
%A Heng Zhu
%A Hengli Tang
%A Hongjun Song
%A Khalida Shamim
%A Kirill Gorshkov
%A Menghang Xia
%A Miao Xu
%A Richard A. Preston
%A Ruili Huang
%A Sergey A. Shiryaev
%A Shihua He
%A Shu Yang
%A Wei Sun
%A Wei Zheng
%A Wenjun Zhu
%A Wenwei Huang
%A Xiangguo Qiu
%A Xin Hu
%A Yi Zhou
%A Yu-Shan Cheng
%A Yuying Wu
%A Zirui Zhang
%J Archive of "Cell Discovery".
%D 2018
%R 10.1038/s41421-018-0034-1
%X a Chemical structures of emetine and cephaeline. b Dose-response curve showing the effect of emetine treatment on ZIKV NS1 protein expression (red) and cell viability (black) in HEK293 cells exposed to ZIKV MR766 strain. c Western blot of ZIKV NS1 protein after treatment of SNB-19 cells with varying concentrations of Emetine. SNB-19 cells were treated with emetine for 1£¿h before inoculation with ZIKV strains PRVABC59, FSS13025, or MR766 (MOI£¿=£¿1), and cells were harvested 24£¿h after infection for Western Blot analysis. d Immunofluorescent images of astrocytoma cells stained for ZIKV PRVABC59 envelope (ENV) protein (green), nuclei (blue) and treated with emetine. Scale bar=100£¿¦Ìm. e Dose¨Cresponse curves from cells in (d) showing the inhibition effect of emetine on ZIKV PRVABC59 infection measured by ENV+£¿SNB-19 cells (green), and cell number by nuclear counting (blue). f SNB-19 cells were treated with emetine at increasing concentrations for 1£¿h before infection with PRVABC59 at a MOI£¿=£¿1 (n£¿=£¿3 cultures). Cell culture supernatant was collected 24£¿h after infection, and infectious virions were quantified for focus-forming units (FFU) using Vero cells. All curves represent best fits for calculating the IC50 values (graph inset). All values represent mean£¿¡À£¿SD (N£¿=£¿3 replicates
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5986771/