%0 Journal Article
%T Smad7:¦Â-catenin complex regulates myogenic gene transcription
%A John C. McDermott
%A Soma Tripathi
%A Tetsuaki Miyake
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1615-0
%X a C2C12 myoblasts were cultured in growth media (GM) for 24£¿h, followed by differentiation media (DM) for designated times. Lysates were collected and assessed for the expression of muscle markers by western blot analysis. b Lysates were combined from several plates for immunoblotting detection of Smad7. Actin was used as loading control. c Co-localization of Smad7 and ¦Â-catenin was analyzed by immunofluorescence in ectopically expressing EYFP-Smad7-NLS and mcherry- ¦Â-catenin C2C12 cells. d Cytoplasmic and nuclear extraction was done to determine the endogenous localization of Smad7 and ¦Â-catenin in C2C12 cells. Extracellular signal-regulated kinase was utilized as cytoplasmic control and c-Jun as nuclear control. e Co-immunoprecipitation (Co-IP) assays were performed using Smad7 antibody to detect an interaction between endogenous ¦Â-catenin and Smad7 in extracts from mouse tibialis anterior (TA) muscle and C2C12 myoblasts. f Alternatively, in another separate experiment, C2C12 myoblasts were transiently transfected with Smad7-Flag. Smad7-Flag lysates were utilized for Co-IP with ¦Â-catenin or Flag antibody to detect interaction with ¦Â-catenin. IP with IgG served as control
%K DNA
%K Transcriptional regulatory elements
%K Stem-cell research
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522533/