%0 Journal Article
%T Angiomotin-p130 inhibits ¦Â-catenin stability by competing with Axin for binding to tankyrase in breast cancer
%A Fan Wang
%A Jiao Yang
%A Jin Yang
%A Peijun Liu
%A Xiaoman Zhang
%A Yanwei Shen
%A Yaochun Wang
%A Yu Liu
%A Zheling Chen
%J Archive of "Cell Death & Disease".
%D 2019
%R 10.1038/s41419-019-1427-2
%X a Expression levels of Amot-p130 in nine breast cancer cell lines and two immortalized breast epithelial cells (MCF10A and MCF12A) (using 293T cells as the positive control) as determined by (upper) RT-qPCR and (lower) western blotting. GAPDH was used as the loading control. b Interference efficiency of Amot-p130 was evaluated by (upper) RT-qPCR and (lower) western blotting. GAPDH was used as the loading control. c Cell proliferation was measured using cell count assay. d Cell proliferation was measured using plate clone formation. Clones were stained with 0.4% crystal violet (left). Numbers of clones are shown as the mean£¿¡À£¿SD of three independent experiments (right). e Cell cycles were determined by flow cytometry (upper). The proportion of cells distributed in each cell cycle was calculated from three independent experiments (lower). f Cell apoptosis was determined by flow cytometry (left). Numbers of apoptotic cells were calculated from three independent experiments (right). MM231 is the abbreviation of MDA-MB-231. All P values were calculated by paired Student¡¯s t test. *P£¿<£¿0.05, **P£¿<£¿0.01, ***P£¿<£¿0.001. RT-qPCR, real-time quantitative PC
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385204/