%0 Journal Article
%T Single-cell imaging and transcriptomic analyses of endogenous cardiomyocyte dedifferentiation and cycling
%A Amy M. Martinson
%A Avin Mehri
%A Naima Alver
%A Ning Li
%A Nuria Gago-Lopez
%A William Robb MacLellan
%A Yiqiang Zhang
%A Yonggang Liu
%A Zhenhe Zhang
%J Archive of "Cell Discovery".
%D 2019
%R 10.1038/s41421-019-0095-9
%X a Cardiomyocyte renewal can potentially originate from pre-existing cardiomyocytes (GFP+) or resident progenitors (RFP+) in tamoxifen-treated bi-transgenic ¦ÁMHC-MCM;RFPfl/GFP mice. b ¦Á-sarcomeric actinin (¦ÁSA, magenta) immunostaining on myocytes isolated from bi-transgenic ¦ÁMHC-MCM;RFPfl/GFP mice with tamoxifen (TAM) or vehicle (VEH) treatment, or cells from wild-type (WT) littermates. Scale bar£¿=£¿50£¿¦Ìm. c Flow cytometry analysis showing the expression of GFP and RFP in myocytes isolated from hearts of bi-transgenic mice without (VEH) or with tamoxifen (TAM) treatment. The far-left panel shows the total ventricular populations containing small cells (non-myocytes), and larger cells (circled) that were either RFP+ (VEH; 2nd dot plot) or GFP+ (TAM; 3rd dot plot). n£¿=£¿3 mice for each group. d Expression profile of GFP and RFP in cardiomyocytes from bi-transgenic mouse ventricles 10 days, 3.5 weeks, or 3 months after MI or Sham operation. Statistics: p£¿>£¿0.05 in two-way ANOVA analysis (n£¿=£¿3¨C4 mice for each group). e ImageStream analysis on total ventricle cells from 3.5-week post-MI or sham mice. GFP and RFP are shown in channel Ch02 and Ch04, respectively; and BrdU incorporation signal revealed by Alexa Fluor 647-conjugated antibody is shown in Ch05; nuclear staining (by DAPI) in Ch01; and the bright phase signal in Ch03. n£¿=£¿3 mice (Sham or MI). *p£¿<£¿0.05 in t-tes
%K Mechanisms of disease
%K Cell growth
%K Cell growth
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547664/