%0 Journal Article
%T Octopamine mediates protein-seeking behavior in mated female Drosophila
%A Liming Wang
%A Yinjun Tian
%J Archive of "Cell Discovery".
%D 2018
%R 10.1038/s41421-018-0063-9
%X a Schematic illustration of the DAMS-based locomotion assay. Briefly, virgin female flies were collected shortly after eclosion and raised in the presence of sucrose and yeast extract (Sˋ+ˋYE) for 3每5 days. Afterwards, female flies were either mated with males for 1 day or kept as virgins. These mated or virgin females were then transferred individually to polycarbonate tubes (5ˋmm (D)ˋ℅ˋ65ˋmm (L)) and assayed in DAMS. b Midline-crossing activity in 30-min bins of wild-type Canton-S-mated female flies assayed in the presence of 5% sucrose alone (※S§, light orange) or 5% sucrose plus 2% yeast extract (※Sˋ+ˋYE§, dark orange) (nˋ=ˋ49每53). Yellow bars represent light-on period of 12ˋh in this and all other figures. c Average daily midline-crossing activity of flies assayed in b (nˋ=ˋ49每53). d Average daily midline-crossing activity of flies assayed in b from Day 1 to Day 4 (nˋ=ˋ49每53). e每g Average daily midline-crossing activity of flies assayed on different protein-rich food types (nˋ=ˋ38每41, 58每69, 24每28, respectively). h, i Midline-crossing activity in 30-min bins of wild-type Canton-S male flies (h) and virgin female flies (i) assayed in the presence of 5% sucrose alone (※S§, light orange) or 5% sucrose plus 2% yeast extract (※Sˋ+ˋYE§, dark orange) (nˋ=ˋ44每53). j Schematic illustration of the video recording-based locomotion assay. Briefly, individual flies were introduced into a behavioral chamber in the presence of a small food patch located in the center, and their positions and behaviors were recorded and analyzed by a custom computer program. k Spatial distribution of protein-deprived Canton-S flies assayed in the presence of sucrose (left) or sucrose plus yeast extract (right) (the heat maps showed the average duration for flies to stay in each pixel; nˋ=ˋ13 (left) and 17 (right); for SEM see Supplementary Fig. S5). Color temperature represents average time spent on each pixel for the duration of the assay (11ˋh). l Total walking distance of protein-deprived flies assayed in i (nˋ=ˋ13 and 17). m On-food and off-food walking speed of protein-deprived flies assayed in i (nˋ=ˋ13 and 17). n A summary of the behavioral analysis. Briefly, protein deprivation enhances flies＊ protein-seeking behavior, by increasing their tendency to approach protein-rich food, to reduce their movement during their visits to protein-rich food, and to reduce their willingness to leave protein-rich food. o每v Average daily midline-crossing activity of flies assayed in S and Sˋ+ˋYE food (o, nˋ=ˋ27每42; p, nˋ=ˋ26每32; q, nˋ=ˋ28每39; r, nˋ=ˋ25每33; s, nˋ=ˋ44每55; t, nˋ=ˋ22每32; u,
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6277437/