%0 Journal Article
%T Interfering cellular lactate homeostasis overcomes Taxol resistance of breast cancer cells through the microRNA-124-mediated lactate transporter (MCT1) inhibition
%A Guo-qing Song
%A Lu Hou
%A Si-li Jin
%A Xiao-hu Jin
%A Yi Zhao
%A Yi-chong Chen
%A Yi-han Fang
%A Ying-han Ma
%J Archive of "Cancer Cell International".
%D 2019
%R 10.1186/s12935-019-0904-0
%X Lactate homeostasis is positively correlated with Taxol resistance of breast cancer cells. a BT474, SKBR3 and MCf7 cells were treated with indicated concentrations for 48 h, the protein levels and (b¨Cd) mRNA levels of LHDA and MCT1 were detected by Western blot and qRT-PCR, respectively. e¨Cg Lactate productions of BT474, SKBR3 and MCF7 cells were measured under indicated Taxol treatments. h Cell viability assays were performed in MCF7 parental and Taxol resistant cells under 0, 5, 10, 20, 40, 80, 160 and 320 nM Taxol treatments for 48 h. i Protein and (j, k) mRNA expressions of MCT1 and LDHA were detected in MCF7 parental and Taxol resistant cells by Western blot and qRT-PCR, respectively. ¦Â-actin is a loading control. l Glucose consumption and (M) lactate production were measured in MCF7 parental and Taxol resistant cells. *p£¿<£¿0.05; **p£¿<£¿0.01; ***p£¿<£¿0.00
%K Breast cancer therapy
%K MicroRNA-124
%K Taxol resistance
%K Lactate homeostasis
%K MCT1 inhibitor
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657142/