%0 Journal Article
%T A Basic Study of Photodynamic Therapy with Glucose-Conjugated Chlorin e6 Using Mammary Carcinoma Xenografts
%A Akihiro Nomoto
%A Hiroaki Yamaguchi
%A Hiromi Kataoka
%A Inoru Yokoe
%A Mamoru Tanaka
%A Shigenobu Yano
%A Shota Hibino
%A Tomohiro Osaki
%A Yoshiharu Okamoto
%A Yuji Mikata
%J Archive of "Cancers".
%D 2019
%R 10.3390/cancers11050636
%X By using the Warburg effect¡ªa phenomenon where tumors consume higher glucose levels than normal cells¡ªon cancer cells to enhance the effect of photodynamic therapy (PDT), we developed a new photosensitizer, glucose-conjugated chlorin e6 (G-Ce6). We analyzed the efficacy of PDT with G-Ce6 against canine mammary carcinoma (CMC) in vitro and in vivo. The pharmacokinetics of G-Ce6 at 2, 5, and 20 mg/kg was examined in normal dogs, whereas its intracellular localization, concentration, and photodynamic effects were investigated in vitro using CMC cells (SNP cells). G-Ce6 (10 mg/kg) was administered in vivo at 5 min or 3 h before laser irradiation to SNP tumor-bearing murine models. The in vitro study revealed that G-Ce6 was mainly localized to the lysosomes. Cell viability decreased in a G-Ce6 concentration- and light intensity-dependent manner in the PDT group. Cell death induced by PDT with G-Ce6 was not inhibited by an apoptosis inhibitor. In the in vivo study, 5-min-interval PDT exhibited greater effects than 3-h-interval PDT. The mean maximum blood concentration and half-life of G-Ce6 (2 mg/kg) were 15.19 ¡À 4.44 ¦Ìg/mL and 3.02 ¡À 0.58 h, respectively. Thus, 5-min-interval PDT with G-Ce6 was considered effective against CMC
%K photodynamic therapy
%K glucose-conjugated chlorin e6
%K canine mammary carcinoma
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562844/