%0 Journal Article
%T Risk stratification of adult T©\cell leukemia/lymphoma using immunophenotyping
%A Charles R. M. Bangham
%A Graham P. Taylor
%A Huseini H. Kagdi
%A Juan Carlos Ramos
%A Maria A. Demontis
%A Paul A. Fields
%J Archive of "Cancer Medicine".
%D 2017
%R 10.1002/cam4.928
%X Adult T©\cell leukemia/lymphoma (ATL), a human T©\lymphotropic virus type 1 (HTLV©\1)©\associated disease, has a highly variable clinical course and four subtypes with therapeutic and prognostic implications. However, there are overlapping features between ATL subtypes and between ATL and nonmalignant (non©\ATL) HTLV©\1 infection complicating diagnosis and prognostication. To further refine the diagnosis and prognosis of ATL, we characterized the immunophenotype of HTLV©\1©\infected cells in ATL and non©\ATL. A retrospective study of peripheral blood samples from 10 HTLV©\1©\uninfected subjects (UI), 54 HTLV©\1©\infected patients with non©\ATL, and 22 with ATL was performed using flow cytometry. All patients with ATL had CD4+ CCR4+ CD26£¿ immunophenotype and the frequency of CD4+ CCR4+ CD26£¿ T cells correlated highly significantly with the proviral load in non©\ATL suggesting CD4+ CCR4+ CD26£¿ as a marker of HTLV©\1©\infected cells. Further immunophenotyping of CD4+ CCR4+ CD26£¿ cells revealed that 95% patients with ATL had a CD7£¿ (¡Ü30% CD7+ cells), whereas 95% HTLV+ non©\ATL had CD7+ (>30% CD7+ cells) immunophenotype. All patients with aggressive ATL had a CCR7+ (¡Ý30%), whereas 92% with indolent ATL and 100% non©\ATL had a CCR7£¿ (<30%) immunophenotype. Patients with nonprogressing indolent ATL were CD127+ but those with progressive lymphocytosis requiring systemic therapy had a CD127£¿ (¡Ü30%) immunophenotype. In summary, HTLV©\1©\infected cells have a CD4+ CCR4+ CD26£¿ immunophenotype. Within this population, CD7£¿ phenotype suggests a diagnosis of ATL, CCR7+ phenotype identifies aggressive ATL, while CCR7£¿ CD127£¿ phenotype identifies progressive indolent ATL
%K Adult T©\cell leukemia/lymphoma (ATL)
%K chemokine receptor(s)
%K human T©\lymphotropic virus type 1 (HTLV©\1)
%K immunophenotyping
%K interleukin receptor(s)
%U https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5269699/